Vet-o-test
VET513
FIP Ab
Only for export!
Introduction
Feline Infectious Peritonitis (FIP) is a disease caused by corona virus, actually there are hundreds of different corona viruses but only 7-8 strains of them produce FIP. With the assistance of the antibodies that are supposed to protect the cat, white blood cells are infected with virus, and these cells then transport the virus throughout the cat's body. An intense inflammatory reaction occurs around vessels in the tissues where these infected cells locate, often in the abdomen, kidney, or brain. It is this interaction between the body's own immune system and the virus that is responsible for the disease. Once a cat develops clinical FIP involving one or many systems of the cat's body, the disease is progressive and is almost always fatal. The way clinical FIP develops as an immunemediated disease is unique, unlike any other viral disease of animals.
Principles
Vet-o-test FIP Ab is designed to detect the antibodies of Feline Infectious peritonitis Virus in feline whole blood, serum or plasma. After being absorbed into the cellulose pad, the antibodies of FIP move and bind with gold-colloid complex of Feline Infectious Peritonitis Virus N protein antigen of the conjugate pad, forming Ag-Ab complex. This complex, then, forms Ag-Ab-Ag direct sandwich binding with N protein antigen (Ag) of FIP in the nitrocellulose membrane. The test results can appear on Control (C) and Test (T) lines where the principles of immunochromatography are used.
Characteristics
One-step rapid test of Feline Infectious Peritonitis Virus antibodies
Rapid test results between 5 ~ 10 minutes
Expensive equipment not required
Easy storage and maintenance
High-purity and high-quality materials of the test kit increase its sensitivity and specificity.
Materials (10 tests/kit)
Test 10 units
Diluent (buffer) 2 ㎖ x 1 unit
Disposable dropper for sample collection 10 units
Instruction for use 1 unit
Composition
Specimen well (S: for dropping), Test line (T), and Control line (C) are marked on the device. Inside it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test paper), and absorbent pad
Effect
Detection of anti-Feline Infectious Peritonitis Virus antibody from feline whole blood, serum, or plasma
Uses
Specimen
Feline whole blood, serum or plasma
Test procedure
a) When specimen and test kits are stored at cold circumstances (2~8 ℃), put them at room temperature for 15~30 minutes before use.
b) Take out a device from a pouch and place it on a horizontal surface.
c) Using a dropper as a pipette, obtain the specimen and dispense 1 drop (40 ㎕) of the fluid into the specimen well (S).
d) When the specimen is completely absorbed into the specimen well (S), drop about 1 drop (40 ㎕) of buffer.
e) Read the test results between 5 ~ 10 minutes.
Consider the test results as invalid after 10 minutes.
Interpretation of the results
A purple band should appear on the control line regardless of the test result. The presence of another band on the test line determines the result.
Control Line (C): The line should always appear regardless of the presence of the antibodies of FIPV. If this line does not appear, the test should be considered invalid.
This is probably because of impure buffer and the lack of specimen. It should be tested again with new buffer.
Test Line (T): The presence of antibodies of FIPV determines the test line.
1) Negative: Control line only appears.
2) Positive: Both Test and Control lines appear.
3) Retesting
a) Both Test and Control lines do not appear.
b) Test line only appears.
FIP Positive (+):
A positive result means 1) animal has exposed FCoV or 2) has infected with FCoV and maybe capable of transmitting virus.
FIP Negative (-):
A negative result indicates that 1) the cat has not been exposed and 2) the cat has no antibody to FIPV.
Further examinations
This test is for primary screening. Consult veterinarians for further necessary examinations to obtain clinical test results.
Precautions
Use for Feline In-vitro diagnostic purposes only.
Use within 10 minutes after opening the pouch because the test kit is very sensitive to moisture and its effect may diminish.
Be careful of not touching the result window.
Every specimen should be used with different droppers.
For testing, buffer included only should be used.
Do not use specimen showing hemolysis or being contaminated with microbes, which may cause false-positive or negative result.
Deal with specimen carefully. They can deliver unknown virus or infectious bacteria.
Use disposable gloves when you suspect the infection caused by specimen. And wash your hands later.
Dispose solid wastes after sterilizing them at 121℃ for over 1 hour.
Do not use the kit when its pouch is torn, sealing is not good shape and expiration date passes.
Storage method
The test kits store at 2~30℃ can be used for 18 months after manufacturing. Do not keep them in refrigerator. However, if they are stored under cold circumstances, keep them at room temperature for 15 ~ 30 minutes before use.
Exchange
The test kits are manufactured under strict quality control system. Nevertheless, if they deteriorate during delivery, ask our distributors for exchange.
Only for research purposes.
